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Title: CA1 Pyramidal Neurons Labelled with DiI
Author: Hannah Reid
Votes: 79

Views: 313
Description: Rat brain tissue collected by rapid decapitation followed by compresstome slicing at 200 um. Tissue slices post-fixed in 1% PFA for 1 hour before transfer to PBS. DiI crystals were placed in the CA1 of the hippocampus using a micromanipulator under 2x magnification. Tissue incubated with crystals for 24 hours before a removal and incubation with 1:15000 Hoecsht for 20 minutes and coverslipped with flurormountG. Images aquired on an Olympus FV1000 confocal microscope. Neurons imaged are CA1 pyramidal neurons, with Hoecsht counterstain showing cell nuclei.

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