February Image Contest

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Title: Flowering of Neuronal Activity
Author: Karthik Krishnamurthy
Votes: 0

Views: 249
Description: Cortical neurons cultured from rat cortex for 7 days in vitro and labeled with the calcium indicator Fluo-4. Neurons were then stimulated with glutamate and Fluo-4 fluorescence intensity was imaged for 30 minutes at 5 second intervals using a 20X air objective of a nikon Confocal microscope (A1R). Image represents volume view of fluo-4 fluorescence intensity indicative of calcium influx over the 30 minute period blended using depth color coded alpha algorithm as a function of time.