February Image Contest

Previous photoNext photo
Contest is finished!
Title: Flowering of Neuronal Activity
Author: Karthik Krishnamurthy
Votes: 4

Views: 234
Description: Cortical neurons cultured from rat cortex for 7 days in vitro and labeled with the calcium indicator Fluo-4. Neurons were then stimulated with glutamate and Fluo-4 fluorescence intensity was imaged for 30 minutes at 5 second intervals using a 20X air objective of a nikon Confocal microscope (A1R). Image represents volume view of fluo-4 fluorescence intensity indicative of calcium influx over the 30 minute period blended using depth color coded alpha algorithm as a function of time.